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. 2016 Oct 6;7:12941. doi: 10.1038/ncomms12941

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Copyright © 2016, The Author(s)

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The 80S ribosome pauses when encountering the UGA codon. The ternary eEFSec:GTP:Sec-tRNA^Sec complex and SBP2 (grey sphere) bind to the ribosome through interactions with the SECIS element, which is in the 3′UTR of the selenoprotein mRNA. After codon recognition, eEFSec hydrolyses GTP and D1 (shades of blue) and D2 (red) move in a ratchet-like motion towards and away from the tRNA-binding (or ventral) side, respectively. Also, the domain D4 (orange) swings ∼26° away from the ventral side. These movements are emphasized in the boxed inset; the GTP- and GDP-bound states of eEFSec are shown in solid and dashed lines, respectively. After eEFSec:GDP dissociation, Sec-tRNA^Sec accommodates and formation of the peptide bond occurs. Note: the variable arm of tRNA^Sec (pointing to the right), D4 of eEFSec, SBP2 and SECIS are most likely oriented perpendicularly to the plane of the paper and towards the reader.