Figure 7. The accumulative mutation of tRNA interacting elements with core Triple mutations in AgnB2 increases the enzyme's affinity to TM84.

ITC results depicted in graphical form compare the TM84-binding affinities of wt AgnB2 and Triple AgnB2 mutant±Q413R or LS domain to (a) TM84 alone; or (b) TM84 in the presence of tRNA^Leu. Error bars represent standard deviation from three independent experiments.