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. 2016 Oct 7;7:13026. doi: 10.1038/ncomms13026

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Copyright © 2016, The Author(s)

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(a) Colour of the seeds from A. thaliana transgenic lines expressing CYP98A8/A9 genes under control of the A. thaliana C4H promoter. Only expression of lineage I CYP98A9 leads to a transparent testa (tt) phenotype. Col-4: wild-type Arabidopsis Columbia-4. Ler: wild-type Arabidopsis Landsberg erecta. Ws: wild-type Arabidopsis Wassilewskija. tt4-1: transparent testa 4 mutant for the chalcone synthase gene. (b) In vitro enzyme assays showing the CYP98A9-dependent conversion of naringenin into eriodictyol. Assays with (+) and controls without (−) NADPH were performed. Eriodictyol (grayed elution time) was detected using the m/z 289.4>153.0 transition in multiple reactions monitoring (MRM) positive mode. (c) Relative naringenin hydroxylase activity (% relative to highest activity set to 100). Error bars represent the s.e. from three independent assays. Aa, Aethionema arabicum; At, Arabidospsis thaliana; Es, Eutrema salsugineum.