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. 2016 Oct 7;7:13079. doi: 10.1038/ncomms13079

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Copyright © 2016, The Author(s)

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(a) Silk proteins as dual-tone bio-resist in EBL. (b) SEM images of nanopatterned crystalline silk as positive or negative resist on the same substrate (due to different structural transitions) depending on ebeam dosages. Scale bar, 5 μm. (c) Schematic illustration of β-sheet-oriented structural transitions regulated by electron energies. (d) Schematics of nanoscale IR spectroscopic imaging using scattering-type SNOM (s-SNOM). (e) Topography of silk nano-aggregates (β-sheet rich) on a silicon substrate. (f,g) Near-field IR phase images at 1,631 and 1,710 cm⁻¹, respectively. (h) Local IR absorption spectra (symbols) depicting the normalized near-field phase signal of crystalline silk by sweeping the output wavenumber of the QCL and using IR nano-spectroscopic imaging.