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. 2016 Oct 7;7:13031. doi: 10.1038/ncomms13031

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Copyright © 2016, The Author(s)

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(a) Representative images of nuclei hybridized with intronic smFISH probes for COOLAIR (green) and FLC (red) showing mutually exclusive transcription. Plants were given no cold (non-vernalized plants (NV)) or different weeks of cold (1W, 2W). DNA labelled with DAPI (blue). Scale bar, 5 μm. (b) Frequency of loci transcribing FLC, when COOLAIR is not present (S) or is detectable at the same locus (S if AS). FLC and COOLAIR transcription are anti-correlated at the level of the gene throughout all time points analysed (NV, 1W, 2W). N=number of cells analysed. All error bars are +/−s.e.m. (c) Representative image of a nucleus hybridized with intronic smFISH probes against non-spliced COOLAIR (green) and FLC (red) in plants exposed to 1-week cold (1W), showing transcription of FLC and COOLAIR from different alleles in the same cell. Scale bar, 5 μm.