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. 2016 Oct 7;7:12963. doi: 10.1038/ncomms12963

Figure 4. ARHGAP36 downregulates PKAC levels.

Figure 4

(a) MDCK cells transfected with YFP-ARHGAP36, or a YFP control, were fixed after 8 or 24 h and subjected to immunofluorescence using antibodies against GFP and PKAC. Images were collected by confocal microscopy. Scale bars, 10 μm. (b) HEK293T cells were transfected with YFP-ARHGAP36 or YFP-Cherry control for 24 h. Lysates were immunoblotted with the indicated antibodies. (c) HEK293T cells were transfected with YFP-ARHGAP36 or YFP control. Lysates were immunoblotted with antibodies against RIα, RIβ RIIα, RIIβ, GAPDH and GFP. (d) MDCK cells transfected with CFP-PKI were fixed after 24 h and subjected to immunofluorescence as in a. Scale bar, 10 μm. (e) MDCK cells transfected with YFP-ΔN (195–516), YFP-N (1–194), YFP-N2 (118–195), YFP-RRV or Cherry-36i were fixed after 24 h and subjected to immunofluorescence as in a. 36i signal was not amplified using antibodies. Scale bars, 10 μm.