Figure 1.

Early Growth Response at 7 d Showing Morphogenic Stimulation of Non-Cell-Autonomous Wus2 and Cell-Autonomous Bbm Gene Delivery into the Scutellum of 18-DAP Embryos.

(A) Introduction of Ubi_pro:moGFP:pinII alone (control). Single green fluorescent cells were observed on the scutellum surface.

(B) to (D) nos_pro:Wus2:pinII cobombardment with the moGFP cassette. Foci of fluorescing cells appeared to enlarge slightly but remained confined to the tips of elongating protrusion (B) or formed a file of fluorescing cells from the tip as the protrusions continued to elongate (arrows in [C] and [D]).

(D) A light micrograph and epifluorescence micrograph from the cyan filter set were superimposed onto each other with no other changes made to the data.

(E) Ubi_pro:Bbm:pinII cointroduced with moGFP. Green fluorescent multicellular clusters were observed.

(F) Cobombardment of the Wus2, Bbm, and moGFP expression cassettes. High growth stimulation was observed with a mixed phenotype exhibiting attributes from both Bbm and Wus2.