Figure 3.

nos_pro:Wus2:pinII Plus Ubi_pro:Bbm:pinII Containing T-DNA Used for DNA Gel Blot Analysis in the T1 Generation.

Red opposing arrows indicate approximate locations of qPCR primers for YFP and moPAT for copy number determinations and PCR primers to detect the absence of the Bbm cDNA sequence and the formation of the newly formed junction across the one remaining loxP site after excision. Locations of EcoRI (red numbers on top) and HindIII (black numbers on bottom) restriction sites used in DNA gel blot analysis are also shown.