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. 2016 Aug 24;28(9):2060–2078. doi: 10.1105/tpc.16.00281

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© 2016 American Society of Plant Biologists. All rights reserved.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at www.plantcell.org/site/license.

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Figure 4. — Gene Structure, Expression Pattern, and Subcellular Localization of Bnams4^b.

(A) The structure of Bnams4^b consists of four parts: portions of three genes and a segment (black dashed line) with no homologous sequences in four reference genome sequences.

(B) Gradient expression of Bnams4^b in leaves with varying degrees of albino phenotypes in Bnams4^b transgenic B. napus. Albino leaves had the highest expression level while green leaves almost had no expression of Bnams4^b. Immunoblotting of Bnams4^b showed the same tendency as transcript levels. Error bars indicate sd. The Duncan’s multiple-range test (P < 0.05, SPSS version 19.0) was used to investigate the significant differences of average relative expressions of the five samples. Different numbers of asterisks between two random samples indicated they have significant difference with means of relative expressions; same numbers mean no significant difference between them.

(C) RT-qPCR analysis of Bnams4^b in sterile plants of NIL 7365AC. RL, rosette leaves; CL, cauline leaves; ST, stems; FL, flowers; Bud0, bud of 0 to 1 mm; Bud1, bud of 1 to 2 mm; Bud3, bud of 3 to 4 mm; Bud4, bud of 4 to 5 mm; SD5, siliques 5 d after pollination; SD15, siliques 15 d after pollination; SD30, siliques 30 d after pollination. Error bars indicate sd. The Duncan's multiple-range test (P < 0.05, SPSS version 19.0) was also used to investigate the significant differences between various tissues.

(D) GUS analysis of Bnams4^b in transgenic wild-type A. thaliana.

(E) Subcellular localization of Bnams4^b protein in A. thaliana leaf protoplasts. For the five images above, from left to right, the GFP signal of AA1-GFP fusion construct, blue fluorescence which was transformed from the chlorophyll red autofluorescent signal, red fluorescent signal of endoplasmic reticulum marker, the images in bright field, and merged images of the above all. Five images below indicated the sublocalization of (AA1+AA2)-GFP fusion construct with the same order of notes as Bnams4^b AA1-GFP fusion construct. Bars =10 μm.