Figure 6.

Ectopic Expression of BKn3 Leads to the Expression of HvSoPIN1, HvNAM, and HvLAX1.

(A) to (H) RNA in situ hybridization of HvSoPIN1 ([A] and [E]), HvNAM ([B], [C], [F], and [G]), and HvLAX1 ([D] and [H]). In the wild type, HvSoPIN1, HvNAM, and HvLAX1 were excluded from the lemma, except in some vascular regions ([A] to [D]). At 120 h, HvNAM was expressed flanking the region of BKn3 expression ([F], white arrowheads). By 170 h, when BKn3 expression was high, all three genes (HvSoPIN1, HvNAM, and HvLAX1) were strongly ectopically expressed in the adaxial half of the Hd lemma ([E], [G], and [H], white arrowheads). Black box, zoomed-in image of the lemma; white dotted line, lemma outline; white arrowheads, ectopic expression. n > 4 for each. Bars = 100 μm.

(I) Approximate times (h) of different events during Hd development.