Figure 3.

Biotinylation Is Not Required for BADC-BCCP Interaction.

(A) Purified recombinant His₆-tagged BCCP2 or apo-BCCP1 was resolved by SDS-PAGE and stained with Coomassie blue or blotted with antibiotin antibody.

(B) Coomassie-stained gels showing the elution fractions of Ni²⁺-NTA-purified protein from E. coli. Input shows that both proteins are expressed. Apo-BCCP1 was strongly present in the elution fraction with His₆-BADC1. Protein identities were confirmed by LC-MS/MS.

(C) Strain AH109 yeast was transformed with bait and prey constructs containing the genes shown and plated on medium lacking Trp, Leu, His, and adenine. Empty vector negative control showed no growth. Sv40 and p53 were used as a positive control. Results shown are representative of three biological replicates, i.e., three separate transformation events.