Figure 5.

TCP4 Induces the YUC-Dependent Auxin Response.

(A) and (B) GUS reporter analysis of 6-d-old seedlings grown without (Mock) or with 12 µM DEX. jaw-D;GR denotes the jaw-D;ProTCP4:mTCP4:GR genotype.

(C) Average hypocotyl length of 7-d-old seedlings grown with or without 12 µM DEX. Error bars indicate sd.

(D) GUS analysis of 6-d-old seedlings grown without (Mock) or with 12 µM DEX.

(E) Relative transcript levels determined by RT-qPCR analysis in 5-d-old seedlings (transcript abundances are provided in Supplemental Table 3). Mock and DEX indicate the absence and presence of 12 μM DEX, respectively. All transcript levels were first normalized to PP2A transcripts and then the test samples were compared with the respective controls. Error bars indicate sd. Asterisk indicates P < 0.05; ns indicates not significant. An unpaired Student’s t test was used. Averages from three independent biological samples are shown.

(F) GUS analysis of representative cotyledon tips from 6-d-old seedlings grown on medium containing the indicated chemicals; 12 µM DEX and 20 µM l-kyn were used.

(G) Hypocotyl length analysis of 8-d-old Pro35S:mTCP4:GR seedlings grown on medium containing mock (gray filled circle) or DEX (black circles) with the indicated concentrations of l-kyn. Error bars indicate sd. Bars in (A), (B), and (D) = 1 mm.