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. 2016 Sep 7;28(9):2147–2160. doi: 10.1105/tpc.16.00428

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Figure 10. — Magnesium Extraction from Chlorophyll in the Chlorophyll-Protein Complex by SGR.

(A) Pigment analysis after incubation of PSI with SGR. PSI was isolated from Arabidopsis and incubated with recombinant GFP, SGR1 with a FLAG-tag (SGR1-FLAG), and SGRL with a FLAG-tag (SGRL-FLAG) for 60 min. Recombinant proteins were prepared by a wheat germ protein expression system and diluted with the same volume of reaction buffer without purification. GFP was used as a negative control because it has similar molecular weight as SGR. After incubation, pigments were analyzed using HPLC. Pigments were detected at 410 nm.

(B) Pigment analysis after incubation of LHCII with SGR. LHCII was isolated from Arabidopsis and incubated with recombinant GFP, SGR1-FLAG, and SGRL-FLAG for 60 min. Recombinant proteins were prepared with a wheat germ protein expression system and diluted with same volume of the reaction buffer without purification. GFP was used as a negative control because it has a similar molecular weight as SGR. After incubation, pigments were analyzed using HPLC. Pigments were detected at 410 nm.