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. 2016 Sep 7;28(9):2147–2160. doi: 10.1105/tpc.16.00428

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© 2016 American Society of Plant Biologists. All rights reserved.

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Figure 5. — SGR1 Functions as a Mg-Dechelatase in Cells.

(A) SGR1 accumulation in the transformants. Inducible SGR1 with a FLAG-tag (SGR1-FLAG) was introduced into wild-type (pOpON:SGR1-FLAG/WT #19) and pph (pOpON:SGR1-FLAG/pph) plants. SGR1- FLAG was induced by DEX application in the transformants. After DEX or mock treatment for 24 h, proteins were extracted from the plants and SGR1 was detected by immunoblotting analysis using an anti-FLAG antibody.

(B) Pigment analysis after SGR1 induction. After DEX or mock treatment for 24 h, pigments were extracted from the plants and analyzed using HPLC. Fluorescence intensity was monitored (410 nm excitation; 680 nm fluorescence).

(C) Pheophytin a contents in the transformants. After DEX or mock treatment for 24 h, pigments were extracted from the plants and the amount of pheophytin a was determined (n = 4 ±sd).