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. Author manuscript; available in PMC: 2017 Sep 2.
Published in final edited form as: Circ Res. 2016 Jul 28;119(6):764–772. doi: 10.1161/CIRCRESAHA.116.308904

Figure 3. Inducible expression of super compliant titin isoforms recovers diastolic function in TtnΔIAjxn mice.

Figure 3

A) Representative image of 1% agarose gel for titin analysis of LV myocardium. Raloxifine results in expression of two large N2BA isoforms (scN2BA1 and 2). B) RBM20 expression in the constitutive and inducible ΔIAjxn/ΔRRM mouse. The top shows example Western blots and the bottom the ratio of mutant RBM20 to WT RBM20 in constitutive and inducible ΔIAjxn/ΔRRM LV samples. C) E/A ratio; D) MVDT: mitral valve deceleration time; E) EDPVR (β): end diastolic pressure volume relationship. Data shown as mean ± SEM. Age at day 0 = 100 ± 5 days; Age at day 28 = 128 ± 5 days for vehicle and treatment groups. Statistical significance calculated with two-way ANOVA and Bonferroni correction (D and E) or t-test (F), (* p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001 as indicated). (Vehicle: DMSO/Saline; Treatment: raloxifene (40 mg/kg), both administered IP daily for 8 days.)