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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biomaterials. 2015 Oct 22;77:255–266. doi: 10.1016/j.biomaterials.2015.10.026

Figure 4.

Figure 4

Image showing the presence of vascular areas via staining of serial sections for human specific CD-31 staining, Hematoxylin & Eosin (H&E) as well as Masson's trichome staining in control (A), no pre-culture (NP) (B), 1 week pre-culture (C), 2 week pre-culture (2P) (D) and 3 week pre-culture (3P) (E) groups. All the groups show large amount of cells except the control group. The CD-31 immunohistochemistry-stained perfused vascular areas (indicated by blue arrow) can be seen via H&E and Masson’s trichrome stain. Masson’s trichrome staining also reveals structures such as collagen fibrils (indicated by red ‘*’ symbol) as well as fibrin (indicated by green ‘#’ symbol). The scale bar in each image represents 70µm.