Figure 3.

Compound 12 inhibition of Bodipy-Cyclopamine (BC) binding to whole cell expressing either Smo WT or Smo D473H. (a) Competitive binding of Bodipy-Cyclopamine (BC) in HEK293T cells transfected transiently with human WT or mutant Smo (D473H) was conducted with various concentrations of compound 12. BC binding (green) is visualized using fluorescence microscopy in a representative field. (b) The concentrations–response curves express the percentage of BC incorporation observed after compound 12 treatment in HEK293T cells transfected with human WT or mutant (D473H) Smo, respectively. Data show the mean±S.D. of three independent experiments. *P<0.05 versus CTR. Quantitative data are the average BC intensity from five independent fluorescence microscopy images