Figure 2.

Overexpression of miR-182 before treatment with 3 μM cisplatin significantly improved hair cell survival. (a–c) Surviving hairs were confirmed by phalloidin and myosin 7a double staining. The stereocilia of many hair cells had been lost and the cuticular plates had shrunk (white arrowhead) after cisplatin treatment (a, b) Residual hair cell numbers at the apex, mid, and base turn in the miR-183 OE, miR-96 OE, miR-182 OE, and control groups 48 h after cisplatin washout (c). (d) Residual hair cell numbers in the miR-182 rescue and control groups. (e) Residual hair cell numbers in the miR-182 knockdown and control groups 48 h after cisplatin washout. Data are shown as means±S.E. Student's t-test and one-way ANOVA followed by Newman–Keuls test, **P<0.01. Scale bar in bottom-right corner: 20 μm. ns, no significance