Figure 6.

Simultaneous detection of apoptotic and gD/HSV-1-positive cells by immunofluorescence analysis of HSV-1-infected U937-pcDNA and U937-DN-IκB cells. (a) Representative microscopy images captured at 48 h p.i., using fluorescent filters optimized for Hoechst-stained nuclei (Hoechst, blue fluorescence, left panel) and surface gD-expressing cells following staining with a mouse anti-gD antibody and an appropriate secondary fluorescein isothiocyanate-conjugated antibody (gD/HSV-1, green fluorescence, middle panel). Images obtained from the same field with different filters were merged (merge, right panel). Fragmented nuclei typical for apoptosis can be observed. Original magnification (630 × ). Scale bar 10 μM. (b)Venn diagrams showing the overlap (grey) of apoptotic (black) and gD (white) positive cells for the same fields of a total of 958 counted U937-pcDNA and a total of 949 counted U937-DN-IκB cells, evaluated at 24 h p.i. Data are collected from one representative experiment