Figure 2.

RBM24 suppresses NPC proliferation, migration and invasion in vitro. (a) Western blotting analysis verifying the overexpression of RBM24 in 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells treated with or without doxycycline. (b) The cell growth rates were determined by CCK8 assay (**P<0.01, Student's t-test). (c) Induction of foci formation and (d) colony formation in soft agar of 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells treated with or without doxycycline. The numbers of foci and colonies were calculated and are depicted in the bar chart. The values indicate the mean standard deviation of three independent experiments (magnification, × 200; **P<0.01, Student's t-test). (e) Transwell assay showing the migration and invasion of 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells treated with or without doxycycline. Migrating and invaded cells were fixed and stained with crystal violet (magnification, × 100). The number of migrating and invaded cells were calculated and are depicted in the bar chart. All data are shown as the mean±S.E.M. of three independent experiments (*P<0.05 and **P<0.01, Student's t-test)