Figure 4.

RBM24 upregulates miR-25 expression. (a) Microarray profiling results showing the miRNAs that were regulated by RBM24. (b) QRT-PCR analysis of miR-25 expression in 5-8 F, CNE-2 and HONE-1 Tet-Off-inducible RBM24-stable cells treated with or without doxycycline (*P<0.05 and **P<0.01, Student's t-test). (c) CCK8 assay of proliferation of 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells that were transfected with miR-25 mimic (50 nM), NC mimic, miR-25 inhibitor (100 nM) or NC inhibitor after treatment with doxycycline or removal of doxycycline for 24 h (**P<0.01, Student's t-test). (d) Quantification of the indicated migrating and invaded cells by transwell analysis for 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells treated as in (c). All data are shown as the mean±S.E.M. of three independent experiments (*P<0.05 and **P<0.01, Student's t-test)