Figure 5.

miR-25 reduced the MALAT1 level. (a) Schematic constructions of the wild type and mutant pMIR-REPORT-MALAT1 miRNA expression vectors used in luciferase reporter assays. The five altered nucleotides in the mutant binding site are colored in red. (b) The relative luciferase activities in the 5-8 F and CNE-2 cells after transfection with the pMIR-REPORT-MALAT1 reporter and miR-25 mimic, NC mimic, miR-25 inhibitor or NC inhibitor. (c) QRT-PCR analysis of MALAT1 expression in 5-8 F and CNE-2 cells that were transfected with miR-25 mimic (50 nM) or NC mimic. (d) QRT-PCR analysis of MALAT1 expression in 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells that were transfected with miR-25 inhibitor (100 nM) or NC inhibitor after the removal of doxycycline for 24 h. All data are shown as the mean±S.E.M. of three independent experiments (*P<0.05 and **P<0.01, Student's t-test)