Skip to main content
. 2016 Sep 1;7(9):e2352. doi: 10.1038/cddis.2016.252

Figure 6.

Figure 6

RBM24 reduced the MALAT1 level by upregulating miR-25. (a and b) QRT-PCR, revealing MALAT1 and miR-25 expression following RBM24 knockdown in 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells after the removal of doxycycline for 24 h. (c) QRT-PCR analysis of miR-25 and MALAT1 expression in NPEC1 Bmi-1 cells that were transiently transfected with siRNAs (50 nM) against RBM24 or scramble siRNAs as controls. (d) Western blotting analysis of RBM24 expression in 5-8 F and CNE-2 cells that were transfected with RBM24 overexpression vector, RRM deletion vector or control vector for 48 h. (e and f) QRT-PCR analysis of miR-25 and MALAT1 expression in 5-8 F and CNE-2 cells treated as in (d). (g) Western blotting analysis, verifying the knockdown of Ago2 in 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells after the removal of doxycycline for 24 h. (h) QRT-PCR analysis of MALAT1 expression in 5-8 F and CNE-2 Tet-Off-inducible RBM24-stable cells treated as in (g). All data are shown as the mean±S.E.M. of three independent experiments (*P<0.05 and **P<0.01, Student's t-test)