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Cytotoxic effect of JS-K (1–25 μM) and DMSO control (0, 48%) on U87, LN229 and primary IC cells after 48 h (a) and 72 h (b). Cell viability was determined by MTT assay at the end of the incubation time. Cytotoxicity induced by JS-K was plotted relative to viability of untreated controls set to 100% (±s.d. of three independent experiments). Asterisks (*P<0.05, **P<0.01, ***P<0.001) indicate significance between JS-K and controls
