Figure 2.

Effect of OGD/R on ANXA1 accumulation on the Bid gene promoter. (a) Venn diagram of the overlap among all ANXA1-bound genes identified by ChIP-Seq in primary cultured neurons treated without or with OGD/R. The genes that bound ANXA1 from the normal and OGD/R group (n=3 independent samples) are shown. (b) GO enrichment of ANXA1-bound proteins compared with the total genes from the rat genome. Significant enrichments of GO terms were obtained for the biological processes shown. (c) UCSC Genome Browser tracks depicting ANXA1 ChIP-Seq peaks at representative Bid gene loci under OGD/R conditions. (d) ChIP-PCR results show the interaction between ANXA1 and the Bid gene promoter after OGD/R in HEK293 cells. (e) Statistical analysis of the data shown in (d). The data are expressed as the means±S.E.M. from three independent experiments. *P<0.05 versus control