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. 2016 Sep 1;7(9):e2356. doi: 10.1038/cddis.2016.259

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ANXA1 interacts with p53 in the nucleus after OGD/R. (a) Western blot results demonstrating the expression of p53 in the cytoplasm and nucleus in primary cultured neurons treated without or with OGD/R. (b) Quantitative densitometry analysis of the p53 expression shown in (a). The data are expressed as the means±S.E.M. from three independent experiments. *P<0.05 versus control. (c) Representative Co-IP results show the interactions of ANXA1 with p53 in HEK293 cells transfected with pcDNA-ANXA1 (His-tagged) and pEGFP-p53 (GFP-tagged) treated without or with OGD/R. (d) Representative Co-IP results show the interactions of ANXA1 with p53 in primary cultured neurons treated without or with OGD/R. (e) Immunofluorescence shows the colocalization of endogenous p53 and ANXA1 in primary cultured neurons treated with or without OGD/R. Scale bar=50 μm. (f) The nuclear colocalization of endogenous ANXA1 and p53 was quantitated as a graph. The data are expressed as the means±S.E.M. from three independent experiments. *P<0.05 versus control. (g) Immunofluorescence shows the colocalization of ANXA1 and p53 in HEK293 cells transfected with pjRed-ANXA1 and pEGFP-p53 treated with or without OGD/R. Scale bar=50 μm. (h) The nuclear colocalization of endogenous ANXA1 and p53 was quantitated as a graph. The data are expressed as the means±S.E.M. from three independent experiments. *P<0.05 versus control