Figure 2.

Effect of Nrf2 or TLR4 on ADSC-mediated survival of fat grafts. Nrf2^−/− or TLR4^−/− mice were injected subcutaneously in the left flank with fat tissues with or without 0.2 ml of 1 × 10⁷/ml GFP-labeled ADSCs. (a) Fat tissues were excised and representative images are shown. (b) The survival ratio of transplanted fat tissues was calculated using the following formula: survival volume/previous volume (0.5 ml). **P<0.01, ***P<0.001 versus control. (c–e) The expression of NOX1, NOX4 and HO-1 in transplanted adipose tissue 2 weeks after fat graft was measured by real-time PCR. *P<0.05, **P<0.01, ***P<0.001 versus control. (f) Hematoxylin and eosin (HE) staining of fat grafts (magnification, × 400) after 2 months. The arrowheads show the changes in the number of capillaries in the different groups. (g) The number of capillaries was counted from 10 fields per section taken from a central tissue section. **P<0.01, ***P<0.001 versus the adipose tissue group (adipose tissue group: fat graft without added ADSCs). (h) Measurement of ROS in adipose tissues 2 weeks after fat transplantation (n=5). *P<0.05, ***P<0.001 versus adipose tissue group