Figure 3.
Origin of endothelial cells in surviving transplanted fat tissue. GFP-labeled ADSCs were detected by immunohistochemistry in sections from fat transplants. Representative images are shown. Transplant sections were stained with a Cy3-labeled mAb targeted against the endothelial cell marker CD31 (blue fluorescence) or phycoerythrin (red color) targeted against the endothelial cell marker vWF. The merged images show the co-localization of CD31 or vWF with GFP, indicating the differentiation of endothelial cells from GFP-labeled ADSCs (magnification, × 200). The result show that the survive ADSCs can differentiation into vascular endothelial cells in both a and b with different endothelial cell marker staining. Scale bar: 50 μm