Figure 2.

Development of AML in mice transplanted with ATG5^WT and Atg5^FL MLL-AF9-BM cells transduced with dMLL-AF9 and treated with tamoxifen. (a) A schematic representing the strategy by which the role of Atg5-dependent autophagy was assessed during primary transplantation in MLL-AF9-driven AML. (b) The Kaplan–Meier survival curve of mice transplanted with Atg5^WT (n=8) and Atg5^FL (n=7) MLL-AF9 cells. The P-value for the log-rank test between the two groups is shown. (c) Genomic DNA extracted from the splenocytes of a representative Atg5^WT and six Atg5^FL moribund mice from panel (b) were analyzed by PCR for the status of Atg5 alleles. (d) Representative figures of peripheral blood smears stained by May Grünwald–Giemsa (top) and liver section stained by hematoxylin and eosin (bottom) of moribund mice from panel (b). (e) The spleen weight of moribund mice from panel (b) for Atg5^WT (n=7) and Atg5^FL (n=6) mice. Error bars represent S.E.M. (f) The Annexin V⁻7-AAD⁻CD11b⁺ myeloid cells are shown as percentage of all Annexin V⁻7-AAD⁻ cells according to flow cytometry in the indicated hematopoietic tissues of moribund mice from panel (b). Error bars represent S.E.M. of six mice