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. 2016 Sep 8;7(9):e2364. doi: 10.1038/cddis.2016.267

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Long-range interaction between the c-myb promoter and distal regions. 4C assay was performed in M1 cells. A Csp6I fragment of 1364 bps containing the c-myb promoter was used as bait fragment. The bait region was mapped on the read count distribution plot generated by the Seqmonk software to visualize proximity of the peaks to the bait. The cross-linking frequency between the upstream regions and the bait fragment was presented by reads density. Interaction upstream regions of c-myb are marked by red dashed boxes. Distance of marked interaction regions from transcription start site (TSS) is shown as indicated