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. 2016 Sep 8;7(9):e2364. doi: 10.1038/cddis.2016.267

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Enrichment of Cohesin and CTCF at upstream regions of the c-myb locus. ChIP using antibodies specific for Cohesin or CTCF was performed in M1 and differentiated M1 cells induced by IL-6. Primer sets specific for the regions of +2.8k, −25k, −28k, and −56k were used for quantitative PCR of ChIP DNAs. Relative quantitation was carried out by the comparative threshold cycle (CT) method. Statistical analysis was performed using the GraphPad Prism 5 software. Student's t-test was used on measurements of enrichment from three replicates. Error bars represent S.D. (n=3)