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. 2016 Sep 8;7(9):e2364. doi: 10.1038/cddis.2016.267

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Hoxa9 binding at the distal element is associated with c-myb expression. M1 cells were transfected with MIGR-Hoxa9-ER vector and then Hoxa9 activation was induced by addition of 500 nM 4-OHT. The cells were treated with IL-6 or/and 4-OHT for the indicated times, then ChIP-qPCR was performed to investigate the enrichment of Hoxa9 at (a) the c-myb promoter and (b) the −28k region, and (c) the enrichment of H3K36me3 (active transcription marker) at the c-myb promoter. Relative quantitation was carried out by the comparative threshold cycle (CT) method. Statistical analysis was performed using the GraphPad Prism 5 software. Student's t-test was used on measurements of enrichment from three replicates. Error bars represent S.D. (n=3)