Figure 7.

Regulation of c-myb expression by Hoxa9 and PU.1. M1 cells were infected with lentivirus containing (a) shRNA targeting Hoxa9 (sh-Hoxa9) or (b) PU.1 (sh-PU.1) or scrambled RNA (pLKO.1). Forty-eight hours later, mRNA levels of c-myb and Meis1 were determined by quantitative reverse transcription PCR (RT-qPCR). Relative quantitation was carried out by the comparative threshold cycle (CT) method. Data are normalized to GAPDH expression. Statistical analysis was performed using the GraphPad Prism 5 software. Student's t-test was used on measurements of enrichment from three replicates. Error bars represent S.D. (n=3). An asterisk represents significant difference of expression (P<0.05). **P<0.01, ***P<0.001