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Sp1 binds to the promoter of Nanog. (a) Chromatin immunoprecipitation in both HepG2 and HCCLM3 cell lines. (b) Schematic diagram of the dual luciferase protein target reporter vector with wild type (upper panel) or mutant (lower panel) of Nanog and confirmed by DNA sequencing. (c) Luciferase activity was assayed in miR-612-o HCCLM3 cells. Data are mean±S.D. (n=3) and are representative of three independent experiments
