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. 2016 Sep 29;7(9):e2377. doi: 10.1038/cddis.2016.282

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sp1 suppresses the stemness of HCC in vitro. (a) Western blot analysis to evaluate the expression levels of Sp1 in HepG2, HCCLM3 wild-type (WT), negative control (NC) and Sp1 knockdown (shRNA1, shRNA 2, shRNA 3, shRNA4) cells. (b and c) Representative images and statistical results of HepG2 and HCCLM3 tumor spheres (>50 μm) after indicated treatments (n=3; scale bar: 50 μm). (d) Western blot analysis of Nanog, EpCAM, CD133 in HepG2, HCCLM3 wild-type (WT), negative control (NC) and Sp1 knockdown (shRNA4) cells. Statistical analysis by paired t-test (*P<0.05, **P<0.01)