Figure 2.

miR-134 drives a positive feedback loop that amplifies ERK/JNK-AP-1 signaling by targeting SDS22. (a) The luciferase activity of Luc-SDS22 3′-UTR was inhibited in T29 cells transfected with miR-134 mimics (miR-134). (b) SDS22 protein levels in cells transfected with miR-134 mimics or the miR-134 inhibitors (In-miR-134). (c) The levels of p-JNK, p-ERK and Fra-1 in SDS22-knockdown or SDS22-overexpressing cells were analyzed by western blot. (d) The levels of p-JNK, p-ERK and Fra-1 in miR-134-transfected cells. (e) The levels of p-JNK, p-ERK and Fra-1 in cells co-transfected with miR-con/miR-134 and pcDNA3.1/pSDS22 as indicated. (f) Relative AP-1 activity in SDS22-knockdown or SDS22-overexpressing cells. (g) Relative AP-1 activity in T29 cells co-transfected with miR-con/miR-134 and pcDNA3.1/pSDS22 as indicated. (h) The levels of miR-134 in SDS22-knockdown T29 cells and SDS22-overexpressing T29H cells. (i) Fra-1 expression was induced in SKOV3 cells transfected with miR-134 mimics. (j) The relative levels of pri-miR-134 in SKOV3 cells transfected with miR-134 or siFRA-1. (k) The relative luciferase activity of the P1 constructs in miR-134- or siFRA-1-transfected SKOV3 cells. Data are shown as mean±S.D. from three independent experiments. *P<0.05, **P<0.01 by Student's t-test