Figure 2.

Expression and nuclear import of RAD51 is impaired in PLAURsi cells. (a) sicon and PLAURsi, MDA-MB-231 and HeLa cells were synchronized by double thymidine block and treated with Dox (2 μM) for 1 h, and lysates were made at indicated time points. Western blotting was performed to check for the expression of RAD51. (b) MDA-MB-231 and HeLa cells silenced for PLAUR were irradiated at 9 Gy, fixed after indicated time points, and nuclear translocation and foci formation of RAD51 was detected by immunofluorescence. Scale bar 20 μm. (c) Graphs showing mean fluorescence intensity (MFI) of RAD51 in the nucleus