Figure 3.

Phosphorylation of RAD51 in PLAURsi cells is abrogated as a result of impaired checkpoint activation. (a) sicon and PLAURsi MDA-MB-231 cells were irradiated at 9 Gy and fixed after 4 h. Phosphorylation of RAD51 at Thr309 and Tyr315 was evaluated by immunofluorescence, graphs showing the MFI of P-RAD51 in the nucleus (below). Scale bar 20 μm. Data shown as mean±S.D. (b) MDA-MB-231 and HeLa cells silenced for PLAUR were synchronized by double thymidine block and treated with Dox (2 μM) for 1 h, lysates were made at indicated time points. Western blotting was performed to check for the phosphorylation of CHK1 at Ser345