Figure 6.

Cell cycle arrest on DNA damage in PLAURsi cells is dependent on TP53 status. (a and b) Cell cycle analysis of MDA-MB-231 (a) and HeLa (b), synchronized by double thymidine block and treated with Dox (2 μM) for 1 h, cells were fixed at the indicated time points, stained with propidium iodide and at least 30 000 cells were analyzed by FACS. Graphs represent the percentage of cells in different phases of the cell cycle. (c and d) sicon and PLAURsi MDA-MB-231 (c) and HeLa (d) were treated with the indicated concentrations of Dox for 1 h, then trypsinised and plated at low density in 10 cm dishes, colonies formed after 12 days were counted using ImageJ. (e and f) sicon and PLAURsi MDA-MB-231 (e) and HeLa (f) were plated at low density and treated with low concentration of Dox (125 nM) for 1 h, washed and incubated for 3 days followed by modified Giemsa staining. Enlarged cells having nuclear mitotic defects are marked with arrows. Graphs depict the population of cells with mitotic abnormalities (below). Data shown as mean ±S.D.