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. 2016 Sep 29;7(9):e2383. doi: 10.1038/cddis.2016.291

Figure 7.

Figure 7

PLAURsi affects the viability of the cells on DNA damage, only in presence of WT-TP53. (a and b) sicon and PLAURsi MDA-MB-231 cells expressing WT-TP53 and HeLa silenced for TP53, were synchronized by double thymidine block and treated with Dox (2 μM) for 1 h, cells were fixed 8 h after treatment. Cells were stained with propidium iodide and cycle analysis was performed, percentage of cells in the different phases were plotted. MDA-MB-231 (PLAURsi S-phase 32%, PLAURsi+WT-TP53 S-phase 49%); HeLa (PLAURsi S-phase 53.3%, PLAURsi+TP53si S-phase 40.1%). (c) PI staining was performed in sicon and PLAURsi HeLa cells 48 h after Dox treatment. Data shown as mean ±S.D. (d) sicon and PLAURsi and PLAUR overexpressing MCF-7 cells were transduced with lentiviral constructs to silence (WT) TP53, and treated overnight with Dox (2 μM), after 48 h cells were stained with propidium iodide (PI) and acquired by FACS to detect the number of dead cells. Data shown as mean ±S.D.