Table 2.

In vitro cytotoxicity screening with SRB assay upon various extracts of Ruta angustifolia L. Pers against selected cell lines

Cell Line		A549	CasKi	HCT-116	MRC5
Extract	Incubation time	IC50 (μg/ml)
Methanol	72	7.02 ± 0.36	10.56 ± 0.09	14.3 ± 0.93	>100
	48	51.2 ± 1.85	35.00 ± 1.13	49.9 ± 0.36	>100
	24	85.60 ± 0.85	>100.0	14.3 ± 0.93	>100.0
Hexane	72	33.1 ± 1.667	41.20 ± 0.23	49.9 ± 0.36	>100.0
	48	>100.0	>100.0	>100.0	>100.0
	24	>100.0	>100.0	36.40 ± 2.01	>100.0
Chloroform	72	10.1 ± 0.35	9.38 ± 0.49	88.0 ± 2.65	>100.0
	48	22.67 ± 4.31	29.00 ± 0.46	>100.0	>100.0
	24	72.0 ± 0.5	>100.0	11.8 ± 0.1	>100.0
Ethyl Acetate	72	35.1 ± 1.00	18.10 ± 0.15	12.40 ± 0.85	>100.0
	48	>100.0	46.00 ± 0.76	27.1 ± 0.21	>100.0
	24	>100.0	>100.0	23.2 ± 0.42	>100.0
Water	72	73.9 ± 8.30	92.2 ± 2.58	>100.0	>100.0
	48	>100	>100.0	>100.0	>100.0
	24	>100	>100.0	>100.0	>100.0
Chloroform without chlorophyll	72	8.8 ± 0.32	13.9 ± 1.23	10.8 ± 0.7	65.7 ± 0.5
	48	23.20 ± 0.90	22.1 ± 0.21	14.3 ± 0.45	>100.0
	24	88.0 ± 0.5	>100.0	21.9 ± 0.46	>100.0

Tabulated values are mean ± standard deviation (SD) of three replicates