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. 2016 Oct 12;9:544. doi: 10.1186/s13071-016-1831-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunoblots of native and recombinant Der g 10 and Der g 11 from Dermanyssus gallinae. a Tropomyosin. b Paramyosin. For both immunoblots: Lanes 1 and 6: Water soluble proteins; Lanes 2 and 7: Membrane associated proteins; Lanes 3 and 8: Integral membrane proteins; Lanes 4 and 9: Insoluble proteins; Lanes 5 and 10: Recombinant protein; Lane 11: Recombinant protein. Lanes 1–5 were probed with IgY extracted from egg yolk from hens immunised with either recombinant Der g 10 (a) or Der g 11 (b), and Lanes 6–10 were probed with control sera. Lane 11 was stained with SimplyBlue safe stain (Invitrogen). Lane M: SeeBlue plus 2 molecular mass markers, in kDa (Invitrogen). Stars indicate heavy and light chains of IgY in Lanes 1 and 2 for a, and 1, 2, 6 and 7 for b