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. 2016 Oct 12;16:130. doi: 10.1186/s12876-016-0537-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Histological findings. a. Hematoxylin and eosin (HE) staining. b. High magnification image of the boxed area. c. HE, p53, and Ki-67 stainings of the border between the hyperplastic glands and the tumor glands. The tumor lesions were strongly positive for p53 and Ki-67. d. Submucosal invasion in the stalk, with an invasion depth of 300 μm (MM, muscularis mucosae; SM, submucosal layer). e. HE, cluster of differentiation 34 (CD34), D2-40, and keratin staining. In CD34-negative and D2-40-positive lymph ducts, keratin-positive cells with acidophilic cytoplasm and large nuclei were observed, indicating lymphatic invasion (arrows)