Figure 1.

Increased retention of mitochondria in CD71 and Ter119 positive erythrocytes in NF-E2 tg mice. Whole blood or bone marrow from wt and tg animals, as indicated, was stained with CD71 and Ter119 antibodies as well as MitoTracker green (MTG) dye. (A, D, F) Representative FACS analysis and gating strategy for one wt and one tg animal for (A) CD71 and Ter119, (D) MTG and CD71 and (F) MTG and Ter119. (B, C) Percentages of erythroid populations in (B) PB and (C) bone marrow of NF-E2 tg and wt mice. (E, G) Percentages of (E) MTG⁺/CD71⁺ and (G) MTG⁺/Ter119⁺ stained cells in NF-E2 tg and wt mice. n= 12 wt, n= 21 tg (15 strain “9”, 6 strain “39”). Histograms show mean and SEM. *P<0.05. Statistical significance was calculated by Student’s t-tests.