4% stacking gel
30% Acrylamide/bis-acrylamide	417.5 μl
1 M Tris pH 6.8	1,093 μl
10% SDS	31.3 μl
10% APS (freshly prepared)	25 μl
TEMED	5 μl
H2O	1,570 μl
10% resolving gel
30% Acrylamide/bis-acrylamide	2,085 μl
1 M Tris pH 6.8	2,185 μl
10% SDS	62.5 μl
5 mM Phos-tag™	93.8 μl
10 mM Zn(NO3)2	93.8 μl
10% APS (freshly prepared)	25 μl
TEMED	5 μl
H2O	1,715 μl