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. Author manuscript; available in PMC: 2016 Oct 12.
Published in final edited form as: Cancer Res. 2014 Jan 14;74(6):1822–1832. doi: 10.1158/0008-5472.CAN-13-1839

Figure 3.

Figure 3

Id2-dependent transcriptional regulation of Olig2 involves Notch effector Hey1. A, Olig2 luciferase reporter activity in NSC (v) and NSC (Id2−/−); *, P < 0.01 determined by the t test. Data, mean ± SD of three independent experiments plated in triplicate and normalized to constitutively expressed Renilla luciferase. B, Olig2 luciferase reporter activity in NSC (v) and NSC (Id2); *, P < 0.01 analyzed using a t test. Data, mean ± SD of three independent experiments plated in triplicate normalized to constitutively expressed Renilla. C, Olig2 mRNA during differentiation time course in NSC (v) and NSC (Id2) determined by qPCR; *, P < 0.05 determined by the t test. Data, mean ± SD of two independent experiments PCR amplified in triplicate. D, Olig2 luciferase reporter activity in NSC(v) and NSC (Id2−/−) transfected with vector or NICD; *,P< 0.05. Data, mean±SD of three independent experiments plated in triplicate normalized to constitutively expressed Renilla. E, expression of the Olig2 luciferase reporter transfected with the indicated expression constructs; *, P < 0.05. Data, mean ± SD of three independent experiments plated in triplicate normalized to constitutively expressed Renilla. F, Hey1 ChIP from NSC (v), NSC (Id2−/−), or NSC (Hey1) 3 days after induction of differentiation. ChIP DNA fragments were PCR amplified with primers corresponding to the Olig2 promoter; *, P < 0.01. Data, mean ± SD of five independent experiments. G, Olig2 luciferase reporter expression from NSC (v), Hey1-expressing, or Id2- and Hey1-coexpressing NSCs 3 days after serum-induced differentiation; *, P < 0.01. Data, mean ± SD of two independent experiments plated in triplicate normalized to constitutively expressed Renilla.