Fig. 3.

The effect of arachidonate supplementation on the molecular species of phosphoinositides in MCF10a cells. The five major species of PIP₃ (A), PIP₂ (B), PIP (C) and PI (D) present in MCF10a cells were quantified by HPLC-ESI mass spectrometry. Cells were incubated in complete medium with or without 14.7 μM arachidonate for the indicated times, as described in the methods. Data is shown for each phosphoinositide species corrected for amount of C36:1 phosphatidylserine in the same sample (mean ± SEM, n = 3). Insets show total combined species of phosphoinositides for each condition. aa = arachidonate.