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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: Nat Microbiol. 2016 Aug 8;1(10):16132. doi: 10.1038/nmicrobiol.2016.132

Figure 1. CAF decreases miR-301b, 301a, and 15a expression.

Figure 1

(a) MH-S cell viability was examined by MTT assay after treatment with CAF at different concentrations for 24 h. (b) Expression of miRNAs was measured using miScript PCR assay in MH-S cells with or without CAF treatment (1 mM, 24 h). The lines indicated genes with >4 fold change among subsets. (c) miR-301b expression in MH-S, MLE-12, and BMDM cells was determined by qRT-PCR 24 h post CAF treatment. (d) qRT-PCR validation of miR-301b expression in cells treated with CAF (1 mM). (e) Northern blot showing the expression of miR-301b, 15a, and 301a in cells treated with LPS (100 ng/ml, 6 h). The above data are representative of 3 independent experiments. Panel a, c, d, means±SD from triplicates. (f) miRNA expression in mouse lungs after CAF treatment (50 mg/kg, i.v., n=3, means±SD). *, p<0.05, **, p<0.01. One-way ANOVA with Tukey’s post hoc.