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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: Nat Microbiol. 2016 Aug 8;1(10):16132. doi: 10.1038/nmicrobiol.2016.132

Figure 3. Enforced expression of miR-301b suppresses bacterium-induced anti-inflammatory responses in vivo.

Figure 3

(a) Mice (n=3) were i.v. injected with NS-m or 301b-m (50 μg/mouse, 24 h), then infected with Pa (1×107 CFU), Kp (1×105 CFU) or Sp (5×106 CFU) for 24 h. Lungs from mice infected with Pa as above were collected and homogenized for immunoblotting for IL-4 and TGF-β1. (b) Organs from mice were collected and homogenized for CFU assay. (c) Neutrophils in BAL and blood were counted by H&E staining. (d, e) Lungs were embedded in formalin for H&E staining and lung injuries were double-blind scored. Scale bar=100 μm. Insets showing tissue injury and inflammatory influx. (f) Lungs were embedded in formalin for immunostaining with a Ly6G antibody to show neutrophil infiltration. Scale bar=50 μm. (g, h) After treatment as above, MPO and ELISA assays were performed to test myeloperoxidase activities in lung homogenates or MIP-1α and IL-17A levels in BAL, respectively. Panel a, d, e, f, representative from triplicate samples; b, c, g, h, data are from 3 mice/group, means+SD. *, p<0.05, **, p<0.01. One-way ANOVA with Tukey’s post hoc.