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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: Nat Microbiol. 2016 Aug 8;1(10):16132. doi: 10.1038/nmicrobiol.2016.132

Figure 4. c-Myb is a functional target of miR-301b.

Figure 4

(a) c-Myb 3′UTR contains one predicted miR-301b binding domain with predicted duplex formations between c-Myb 3′UTR (bottom) and miR-301b (middle). The site of target mutagenesis (top) is also indicated. (b) Luciferase reporter assay evaluating c-Mab targeting with the WT or mutated 3′UTR reporter constructs (WT UTR or MUT UTR) of c-Myb in MH-S and MLE-12 cells that were then transfected with NS-m or 301b-m, respectively. (c) Mice (n=3) were i.v. injected with NS-m or 301b-m (50 μg/mouse, 24 h). BMDM were collected for c-Myb mRNA abundance determination at indicated times by qRT-PCR. (d) MH-S and MLE-12 cells were transfected with NS-m and 301-m for 24 h, and c-Myb mRNA was detected at different time points by qRT-PCR. (e) Mice (n=3) were i.v. injected with NS-m, 301b-m, NS-i or 301b-i (50 μg/mouse, 24 h), infected with Pa (1×107 CFU), Kp (1×105 CFU) or Sp (5×106 CFU) for 24 h, and c-Myb mRNA abundance determined by qRT-PCR. Panel b, d, means±SD from triplicate; c, e, Means±SD from 3 mice. *, p<0.05, **, p<0.01. One-way ANOVA with Tukey’s post hoc.